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Sep 13, 2019Decomposition of mannose, glucose, and acetate within a microaerobic membrane reactor: Evidence of redox cycling processes.
We investigated the anaerobic degradation of mannose, glucose, and acetate in a membrane bioreactor (MBR) at a low temperature (15 degrees C) in order to gain insight into the potential of these carbohydrates as electron donors in microbially mediated biogeochemical processes. The utilized substrates were digested in continuous stirred tank reactors (CSTRs) and then transferred to the MBR. The system was operated with an organic loading rate of about 2.9 g COD(matter)/L per day at 15 degrees C. The biodegradation of all three compounds was observed. However, the degradation kinetics of the substrates varied. All three substrates were more readily degraded within the MBR than in the CSTR at 15 degrees C, indicating that electrons were likely transferred to extracellular electron acceptors. We also studied the redox cycling within the MBR by investigating the potential of glucose, fructose, mannose, and acetate as electron donors in addition to hydrolysis. All of these carbohydrates were consumed for bacterial metabolism in the MBR, but methane was also formed. Mannose and acetate were the best electron donors, as indicated by their minimal degradation product formation. All three carbohydrates were consumed for microbial activity and were shown to be potential electron donors in microbially mediated biogeochemical processes in the MBR at 15 degrees C. These results have implications for the distribution of carbohydrate species in the sea and their role in electron transfer and chemical transformations in the subsurface.“I’m feeling really good now. With our game at Liverpool and now we play Napoli, it will be a great challenge for us.”

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